P-141: Impact of Oxidative Stress Induced with BSO on Development and Dif-ferentiation of Ovarian Follicles in Mice
Authors
Abstract:
Background: Whenever the balance between Antioxidants and reactive oxygen species (ROS) is disrupted towards an overabundance of ROS, oxidative stress (OS) occurs. There is evidence that glutathione as an intracellular antioxidant plays an important role against ROS in female reproductive system. BSO is a selective inhibitor of glutathione peroxidas. The aim of the present study is to investigate BSO induced OS effect on development and differentiation of ovarian follicles in mice. Materials and Methods: In this study, 30 female and 15 male mice were used. The female mice were divided into 3 groups of control, sham and experimental ones.Two female mice at their stereos cycle were put with one male mouse in a cage for mating. Observation of vaginal plaque was considered as the first day of pregnancy and the mice on 13th day of pregnancy received 2 mmol/ kg BSO daily until delivery as IP injection. After the pregnancy the 2, 3, 6 and 7 days old new born were sacrificed and their ovaries were prepared for light and electron microscopic studies. The number of follicular nests and diameter of primordial and primary follicles were determined using Motic software and compared with control values. Results: Light microscopy and morphometry showed that follicular nests are distinguished on the 2nd and 3rd days old newborn and follicles on 6th and 7th days. Morphometric studies revealed that the number of follicular nests were similar in control and sham groups, but significantly (p<0.001) reduced in experimental groups in comparison to control group. However, the number and diameters of primordial and primary follicles in experimental and controls were not significantly different. TEM showed that cytoplasmic organelles in experimental group had preapoptotic features. Conclusion: The result indicates that oxidative stress suppresses follicular differentiation at early stages but does not affect the development of already differentiate follicles.
similar resources
FSH and eCG impact follicles development and expression of ovarian FSHR and caspase-9 in mice
The study aimed to investigate the effects of FSH and eCG on the ovarian and follicular development, expression levels of FSHR and caspase-9 of ovaries in vivo. One hundred and five prepuberty mice were allocated into FSH-1, FSH-2, FSH-3, eCG-1, eCG-2, eCG-3 groups and control group (CG). Mice in FSH-1, FSH-2 and FSH-3 were intramuscularly injected with 5, 10 and 20 IU FSH twice (on day 0 and 4...
full textThe effect of BSO-induced oxidative stress on histologic feature of testis: testosterone secretion and semen parameters in mice
Objective(s): Buthionine sulfoximine (BSO) inhibits synthesis of glutathione as the main intracellular antioxidant. The aim of the present study is to investigate the effect of BSO-induced oxidative stress on histological structure of testis, testosterone secretion and semen parameters. Materials and Methods: Thirty male BALB/c mice were divided into 3 groups. In control group, the mice did not...
full textP-123: Impact of Oxidative Stress on Differentiation of Testis in Mice
Background: Antioxidants and reactive oxygen species (ROS) are in balance in the body. Whenever the balance between these molecules is disrupted towards an overabundance of ROS, oxidative stress (OS) occurs. There are evidence that glutathione as an intracellular antioxidant plays an important role against ROS in female reproductive system. Regarding the effect of BSO as a selective inhibitor o...
full textazerbaijans political development after the collapse of soviet union and implication on relation with the islamic republic of iran
در فصل اول این پایاین نامه در خصوص تاریخ کشور اذربایجان قبل و بعد از جدایی این کشور از ایران مورد بررسی قرار گرفته است ودر فصل دوم تحولات سیاسی این کشور بعد از 1991 و در واقع بعد از فروپاشی شوروی و دولتهایی که روی کار امدند در از جمله دولت ابولفضل ایلچی بیگ،دولت حیدر علی اف وبعد از او پسرش الهام علی اف و نگرش هرکدام از این دولتها به سیاسیت خارجی اذربایجان مورد اشاره قرار گرفته است.در ودر فصل سو...
the impact of morphological awareness on the vocabulary development of the iranian efl students
this study investigated the impact of explicit instruction of morphemic analysis and synthesis on the vocabulary development of the students. the participants were 90 junior high school students divided into two experimental groups and one control group. morphological awareness techniques (analysis/synthesis) and conventional techniques were used to teach vocabulary in the experimental groups a...
15 صفحه اولP-58: BSO-Induced Oxidative Stress Affects Testicular Ultrastructure, Levels of Oxidative Markers, Testosterone and Sperm Fertility
Background: GSH is the main intracellular antioxidant and its level is important for protecting cell from oxidative stress. BSO inhibits GSH synthesis and cause oxidative stress. The study is designed to investigate the effect of BSO induced oxidative stress on testicular histology, semen parameters and sperm fertility. Materials and Methods: The adult male mice were divided into two groups of ...
full textMy Resources
Journal title
volume 8 issue 2.5
pages 152- 152
publication date 2014-07-01
By following a journal you will be notified via email when a new issue of this journal is published.
Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023